H3C14 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ChIP, IF, IHC, WB
Recommended Dilution	WB: 1:500 IHC: 1:1000 IF: 1:1000 CHiP: 2-5µg/million cells
Reactivities	C. elegans, Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Histone H3 [ac Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 4 of human Histone H3.2.
Specificity	Anti-Histone H3 [ac Lys4] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	0.68 mg/ml - lot specific
Conjugation	Unconjugated
Storage	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Stability	Expiration date is one (1) year from date of receipt.
Gene Name	histone cluster 2, H3c
Database Link	Entrez Gene 333932 HumanEntrez Gene 126961 Human UniProt ID Q71DI3
Background	In transcription, H3K4Ac is prevalent at the promoters of active genes. Usually, this modification peaks in the cell cycle after dimethylation of H3K9 occurs, which indicates that there are sequential actions of these two modifications. Methylation that occurs on H3K4 concurrently with acetylation seems to act as an adjuster to the activation effects of acetylation. Shugoshin protein cannot bind to the centromere of active cells when H3K4 is acetylated, which reduces dimethylation, and thus slows meiosis and mitosis. Usually, H3K4Ac is a transitional modification, and will become further modified with methylation as transcription progresses, indicating complex transcriptional regulation. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
Synonyms	rabbit anti-Histone H3 Ac Lys4 antibody, H3.3AH3F3H3F3B, H3.3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K4ac
Note	Anti-Histone H3 [ac Lys4] antibody is tested for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
Reference Data
Protein Pathways	Systemic lupus erythematosus