Goat anti-Patched 1 (zebrafish)
Cat# EB07960
Size : 100ug
Brand : Everest Biotech
Code | Name | Applications | Availability | Size | Tested species | Grade |
---|---|---|---|---|---|---|
EB07960 | Goat Anti-Patched 1 (zebrafish) Antibody | Pep-ELISA, IF | 100µg specific antibody in 200µl | Zebrafish |
Target Protein
Principal Names: ptc1, patched 1, ptc-1, etID309849.2, patched1Official Symbol: ptc1
Accession Number(s): NP_571063.1
Immunogen
Peptide with sequence C-QTGSKKEPFNYSQ, from the internal region of the protein sequence according to NP_571063.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Immunofluorescence: This product has been successfully used in IF studies of zebrafish tumors (Ju et al, Mol Cancer. 2009 Jun 25;8:40.; PMID: 19555497). Recommended concentration: 1-3µg/ml.
Species Reactivity
Tested: ZebrafishExpected from sequence similarity: Zebrafish
Specific Reference
This antibody has been successfully used (in IF on Zebrafish tumors) in the following paper:
Ju B, Spitsbergen J, Eden CJ, Taylor MR, Chen W.
Co-activation of hedgehog and AKT pathways promote tumorigenesis in zebrafish.
Mol Cancer. 2009 Jun 25;8:40.
PMID: 19555497
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol