Endothelin BioAssay™ ELISA Kit (Equine)

The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of Equine endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21aa vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21aa vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease.

Cell culture supernates, serum, plasma (heparin or EDTA).

Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Equine Endothelin.

<0.5pg/ml

3.9pg/ml-250pg/ml

Recognizes natural and recombinant Equine Endothelin. There is no detectable cross-reactivity with other relevant proteins.

The Equine Endothelin BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Equine Endothelin has been precoated onto 96-well plates. Standards(Expression system for standard: Equine peptide; Immunogen sequence: C53-W73) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Equine Endothelin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Equine Endothelin amount of sample captured in plate.

96-well plate precoated with anti-Equine EDN1 antibody, 1x plate
Lyophilized recombinant Equine EDN1 standard, 2x1ng/tube
Biotinylated anti-Equine EDN1 antibody, 1x130ul (dilution 1:100)
Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)
Sample diluent buffer, 1x30ml
Antibody diluent buffer, 1x12ml
ABC diluent buffer, 1x12ml
TMB color developing agent, 1x10ml
TMB stop solution, 1x10ml
PBS washing buffer Powder for 1000ml, 1x buffer

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards