CHAPS [75621-03-3]
Cat# HY-15435-1ml
Size : 10mM/1mL
Brand : MedChemExpress
CHAPS, a derivative of Cholic acid, is a zwitterionic detergent for solubilizing membrane proteins. CHAPS is used for stabilization of various protein-DNA complexes and can retain biochemical activity of proteins in solution.
For research use only. We do not sell to patients.
CHAPS Chemical Structure
CAS No. : 75621-03-3
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Other Forms of CHAPS:
- CHAPS hydrate In-stock
Description |
CHAPS, a derivative of Cholic acid, is a zwitterionic detergent for solubilizing membrane proteins. CHAPS is used for stabilization of various protein-DNA complexes and can retain biochemical activity of proteins in solution[1][2]. |
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In Vitro |
CHAPS (0.5 %) is able to stabilize complexes between DNA and DNA-binding factors such as AP-1, SPI, GATA-1 and α-regulated factor ISGF3, and retains their biochemical activity[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
614.88 |
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Formula |
C32H58N2O7S |
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CAS No. |
75621-03-3 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
O[C@@H]1CC[C@]2(C)[C@@]3(06)C[C@H](O)[C@]4(C)[C@@H]([C@H](C)CCC(NCCC[N+](C)(C)CCCS([O-])(=O)=O)=O)CC[C@@]4(06)[C@]3(06)[C@H](O)C[C@@](06)2C1 |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
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Solvent & Solubility |
In Vitro:
H2O : 83.33 mg/mL (135.52 mM; ultrasonic and warming and heat to 60°C) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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Purity & Documentation | |||||||||||||
References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O | 1 mM | 1.6263 mL | 8.1317 mL | 16.2633 mL | 40.6583 mL |
5 mM | 0.3253 mL | 1.6263 mL | 3.2527 mL | 8.1317 mL | |
10 mM | 0.1626 mL | 0.8132 mL | 1.6263 mL | 4.0658 mL | |
15 mM | 0.1084 mL | 0.5421 mL | 1.0842 mL | 2.7106 mL | |
20 mM | 0.0813 mL | 0.4066 mL | 0.8132 mL | 2.0329 mL | |
25 mM | 0.0651 mL | 0.3253 mL | 0.6505 mL | 1.6263 mL | |
30 mM | 0.0542 mL | 0.2711 mL | 0.5421 mL | 1.3553 mL | |
40 mM | 0.0407 mL | 0.2033 mL | 0.4066 mL | 1.0165 mL | |
50 mM | 0.0325 mL | 0.1626 mL | 0.3253 mL | 0.8132 mL | |
60 mM | 0.0271 mL | 0.1355 mL | 0.2711 mL | 0.6776 mL | |
80 mM | 0.0203 mL | 0.1016 mL | 0.2033 mL | 0.5082 mL | |
100 mM | 0.0163 mL | 0.0813 mL | 0.1626 mL | 0.4066 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.