Murine TNF alpha ELISA Kit, 1 x 48-well (pre-coated)

Specifications

Product Data
Format	1 x 48-well (pre-coated)
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3h45min
Signal	Colorimetric
Curve Range	31.25-1000 pg/ml
Sample Type	Serum, Cell culture supernatant
Sample Volume	100 µl
Specificity	Recognizes both natural and recombinant murine TNF-a
Sensitivity	25 pg/ml
Cross Reactivity	No cross reactivity with other murine cytokines
Components	ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
Background	This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Gene Symbol	Tnf
Gene ID	21926
Standard Curve	Representative Standard Curve