Mouse TGF-beta1 ELISA Kit (96-well)

• Aluminium pouches with a Microwell Plate coated with antibody to mouse TGF-β1 (8x12)
• 2 vials mouse TGF-β1 Standard lyophilized, 1000 pg/ml upon reconstitution
• 2 vials concentrated Biotin-Conjugate anti-mouse TGF-β1 antibody
• 2 vials Streptavidin-HRP solution
• 4 bottle Standard /sample Diluent
• 1 bottle Biotin-Conjugate antibody Diluent
• 1 bottle Streptavidin-HRP Diluent
• 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
• 1 vial Substrate Solution
• 1 vial Stop Solution
• 4 pieces Adhesive Films

Transforming Growth Factor-beta (TGF-.) is a pleiotropic cytokine which exists in five isoforms, known as TGF-.1-5. TGF-.1 is the most abundant form in lymphoid organs and is found almost ubiquitously while other isoforms are expressed in a more restricted distribution. The biologically active forms of all isoforms are disulfide-linked homodimers. The heat- and acid- stable monomeric subunits have a length of 112 amino acids. TGF-.1 is produced in very high levels by platelets. Other cellular sources of TGF-.1 include macrophages, lymphocytes, endothelial cells, chondrocytes, and leukemic cells. TGF-.1 secretion can be induced by steroids, retinoids, EGF, NGF, vitamin D3, and IL-1. Activities of TGF-.1 include inhibition of cell growth for inhibitor for normal and transformed epithelial cells, endothelial cells, fibroblasts, neurons, and lymphoid cells and other hematopoietic cell types. TGF-.1 inhibits the proliferation of T cells and NK cells and down-regulates the activities of activated macrophages. TGF-.1 blocks the anti-tumor activity of IL-2-bearing lymphokine-activated killer (LAK) cells. TGF-.1 has a critical role in the development of regulatory T cells. Dendritic cells exposed to tumors have been reported to secrete TGF-.1 and stimulate expansion of naturally-occurring T reg cells. Moreover, TGF-.1 has been shown to act as a costimulatory factor for expression of Foxp3, leading to the differentiation of CD4+CD25+ Treg cells from peripheral CD4+CD25- progeny. TGF-.-induced regulatory T cells have been termed Ti-Treg.