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Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Rabbit Anti-MINA53 Polyclonal Antibody

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Katalog-Nummer CPA3086-200ul

Size : 200ul

Contact local distributor :

Telefonnummer : +1 850 650 7790

Marke : Cohesion Biosciences

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Contact local distributor :

Telefonnummer : +1 850 650 7790

Western blot analysis of MINA53 expression in rat liver (A) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 53 kD)
Immunofluorescent analysis of MINA53 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Name:	Anti-MINA53 Antibody
Cat No:	CPA3086
Source:	Rabbit
Reactivity:	H, M, R
Applications:	WB, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Rabbit polyclonal antibody to MINA53

Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MINA53. The exact sequence is proprietary.

Purification：The antibody was purified by immunogen affinity chromatography.

Clonality：Polyclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)

Gene Symbol：MINA

Alternative Names：MDIG; MINA53; NO52; Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA; 60S ribosomal protein L27a histidine hydroxylase; Histone lysine demethylase MINA; MYC-induced nuclear antigen; Mineral dust-induced gene protein; Nucleolar protein 52; Ribosomal oxygenase MINA; ROX

Entrez Gene (Human)： 84864;

Entrez Gene (Mouse)： 67014;

Entrez Gene (Rat)： 266670;

SwissProt (Human)： Q8IUF8;

SwissProt (Mouse)： Q8CD15;

SwissProt (Rat)： Q8CFC1;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.