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Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Rabbit Anti-Histone H2A Polyclonal Antibody

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Katalog-Nummer CPA6462-50ul

Size : 50ul

Contact local distributor :

Telefonnummer : +1 850 650 7790

Marke : Cohesion Biosciences

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Contact local distributor :

Telefonnummer : +1 850 650 7790

Western blot analysis of Histone H2A expression in U87MG (A), A549 (B), SGC7901 (C), rat lung (D), mouse brain (E) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 14 kD)
Immunohistochemical analysis of Histone H2A staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Name:	Anti-Histone H2A Antibody
Cat No:	CPA6462
Source:	Rabbit
Reactivity:	H, M, R, B, C, Mk
Applications:	WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Rabbit polyclonal antibody to Histone H2A

Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Histone H2A. The exact sequence is proprietary.

Purification：The antibody was purified by immunogen affinity chromatography.

Clonality：Polyclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/200)

Gene Symbol：HIST1H2AG; HIST1H2AI; HIST1H2AK; HIST1H2AL; HIST1H2AM

Alternative Names：H2AFP; H2AFC; H2AFD; H2AFI; H2AFN; Histone H2A type 1; H2A.1; Histone H2A/p

Entrez Gene (Human)： 8329; 8330; 8332; 8336; 8969;

Entrez Gene (Mouse)： 319164; 319165; 319166; 319167; 319170; 319171; 319172; 319191; 665433;

SwissProt (Human)： P0C0S8;

SwissProt (Mouse)： P22752;

SwissProt (Rat)： P02262;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.