Creatinine BioAssay™ Kit

Creatinine is synthesized in the body at a fairly constant rate from creatine, which is produced during muscle contractions from creatine phosphate. In the blood, creatinine is removed by filtration through the glomeruli of the kidney and is secreted into urine. In healthy individuals,
creatinine secretion is independent of diet and is fairly constant. The creatinine clearance test has become one of the most sensitive tests for measuring glomerular filtration rate. In kidney disease, creatinine levels in the blood are elevated, whereas the creatinine clearance rate and
hence the urine levels are diminished. Creatinine test is most widely used to assess kidney function.

Biological samples

Direct Assays: urine, serum, plasma and biological preparations.
Drug Discovery/Pharmacology: effects of drugs on creatinine metabolism.

Detection limit: 0.10mg/dL (8um)

This Creatinine BioAssay™ kit is designed to measure creatinine directly in biological samples without any pretreatment. The improved Jaffe method utilizes picrate that forms a red colored complex with creatinine. The intensity of the color, measured at 510nm, is directly proportional to creatinine concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw sample.

Reagent A, 1x50ml
Reagent B, 1x50ml
Creatinine Standard, 50mg/dL, 1x1ml

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.