Thyroxine ELISA Kit (Cat)

Katalog-Nummer OKCA00259

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityCat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration< 4 Hours
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:194%90-98%
Serum (n=4)1:285%81-90%
Serum (n=4)1:4102%97-106%
Serum (n=4)1:895%90-101%
ELISA Kit PrincipleAviva Systems Biology Thyroxine ELISA Kit (Cat) (OKCA00259) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Cat Thyroxine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Thyroxine and incubated. The Thyroxine found in the sample or standards competes with the biotinylated Thyroxine for limited binding sites on the immobilized anti-Cat Thyroxine antibody. Excess unbound biotinylated Thyroxine and sample or standard Thyroxine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Thyroxine captured in the well and inversely proportional to the amount of Thyroxine which was contained in the sample or standard.
ELISA Kit Range20 ng/mL - 320 ng/mL
ELISA Kit Recovery
Sample 1
Sample TypeAverage RecoveryRange
Serum (n = 5)0.9287-96%
Sample 2
Sample TypeAverage RecoveryRange
EDTA plasma (n = 4)0.9589-99%
ELISA Kit Reproducibility

Mean Intra-Assay Precision %CV: < 15% (n = 20)

Mean Inter-Assay Precision %CV: < 15% (n = 20)

ELISA Kit Component
ComponentAmount
Anti-Thyroxine Microplate96 Well (12 x 8 Well Strips)
Thyroxine Standard 5 x 1 mL
Thyroxine-Biotin Complex1 x 6 mL
Avidin-HRP Conjugate1 x 6 mL
20X Wash Buffer1 x 15 mL
Substrate A1 x 7 mL
Substrate B1 x 7 mL
Stop Solution1 x 7 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates
Sensitivity10 ng/ml
SpecificityThis assay has high sensitivity and excellent specificity for detection of cat T4. No significant cross-reactivity or interference between cat T4 and analogues was observed.
Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between cat T4 and all the analogues, therefore,
cross reaction may still exist.
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolThyroxine
Alias SymbolsT4
Protein NameThyroxine