Bauhinia purpurea agglutinin or lectin (BPA/BPL) is a tetrameric lectin with a molecular weight of 120 kDa. Binding is generally highest for glycoconjugates containing galactosyl (ß-1,3) N-acetylgalactosamine structures but oligosaccharides with a terminal α-linked N-acetylgalactosamine can also bind to BPL. BPA is lactose-specific and elutes with the sugar lactose. It has specificity for blood groups A, B, O (-SA). Treatment of erythrocytes with neuraminidase or trypsin will increase the agglutination reaction, indicating that the receptor is masked by terminal carbohydrates. Makela's group 2 sugars, particularly N-acetyl-D-galactosamine, are potent inhibitors. Although the binding specificity of BPA is similar to that of peanut agglutinin, the tissue staining patterns of these two lectins are distinct. Affinity purified Bauhinia purpurea Lectin (BPL/BPA) (SKU: 21510028) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. This product comes in a stabilized liquid form.

Specifications:

• Source: Bauhinia purpurea alba (Camel's Foot Tree seeds)
• Activity: A, B, O (-SA)
• Protein Concentration (Based on OD 280): 2 mg purified BPL FITC / 1 mL buffer
• Carbohydrate Specificity: Galactosyl (β-1,3) N-acetylgalactosamine
• Inhibitory Carbohydrate: Lactose
• Divalent ions required: None
• Conjugation: FITC
• Fluorescence: Green
• Excitation: Maximum at 495-500 nm
• Emission: Maximum at 514-521 nm

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 °C for short term use. Clarify by centrifugation, if needed.

Application

Blotting, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Glycobiology