Rat AChE (Acetylcholinesterase) CLIA Kit

Katalog-Nummer NB-22-41862-1

Size : 96T

Marke : Neo Biotech

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Telefonnummer : +1 850 650 7790

  • Rat AChE (Acetylcholinesterase) CLIA Kit

General Info

Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This rat AChE kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence

Information

Gene Symbol: Ache
Gene ID: 83817
Uniprot ID: ACES_RAT
Specificity: This kit recognizes Rat AChE in samples. No significant cross-reactivity or interference between Rat AChE and analogues was observed.
Sample Type: Serum, plasma and other biological fluids

Description

Tissue Specificity Has been found in central nervous system and muscle. Found in embryonic liver and spleen but not in adult liver.
Function Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
Protein Name Acetylcholinesterase
Ache
Cellular Localisation Synapse
Secreted
Cell Membrane
Peripheral Membrane Protein
Isoform H: Cell Membrane
Lipid-Anchor
Gpi-Anchor
Extracellular Side
Alternative CLIA Names Acetylcholinesterase CLIA kit
Ache CLIA kit
Ache CLIA kit
Specificity This kit recognizes Rat AChE in samples. No significant cross-reactivity or interference between Rat AChE and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-106 100
EDTA plasma(n=8) 88-99 93
Cell culture media(n=8) 94-107 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.54 1.25 4.99 0.53 1.27 4.71
Standard deviation 0.05 0.09 0.51 0.07 0.09 0.49
CV (%) 9.26 7.20 10.22 13.21 7.09 10.40
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance