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Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Human Apoptosis Array C1

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Katalog-Nummer AAH-APO-1-4

Size : 4SampleKit

Contact local distributor :

Telefonnummer : +1 850 650 7790

Marke : RayBiotech

Contact local distributor :

Telefonnummer : +1 850 650 7790

Human Apoptosis Array C1

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Membrane-based antibody array for detection 43 apoptosis biomarkers, including Caspase 3, Caspase 8, bad, bax, bcl-2, etc.

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Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Species	Human
Number of Targets Detected	43
Quantitative/Semi-Quantitative	Semi-Quantitative
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support	Membrane
Method Of Detection	Chemiluminescence
Design Principle	Sandwich-based
Research Area	Apoptosis, Akt Signaling, IGF Signaling, p53 Signaling

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
bad Gene Symbol: bad Gene ID: 572 Accession #: Q92934 ELISA Kit: RayBio Human BAD ELISA	bax Gene Symbol: BAX Gene ID: 581 Accession #: Q07812	bcl-2 Gene Symbol: BCL2 Gene ID: 596 Accession #: P10415	bcl-w Gene Symbol: BCL2L2 Gene ID: 599 Accession #: Q92843	BID Gene Symbol: BID Gene ID: 637 Accession #: P55957 ELISA Kit: RayBio Human BID ELISA
BIM Gene Symbol: BCL2L11 Gene ID: 10018 Accession #: Q43521	Caspase-3 Gene Symbol: CASP3 Gene ID: 836 Accession #: P42574	Caspase-8 Gene Symbol: CASP8 Gene ID: 841 Accession #: Q14790	CD40 (TNFRSF5) Gene Symbol: CD40 Gene ID: 958 Accession #: P25942 ELISA Kit: RayBio Human CD40 ELISA	CD40 Ligand (TNFSF5) Gene Symbol: CD40LG Gene ID: 959 Accession #: P29965 ELISA Kit: RayBio Human CD40L ELISA
cIAP-2 Gene Symbol: BIRC3 Gene ID: 330 Accession #: Q13489 ELISA Kit: RayBio Human cIAP2 ELISA	Cytochrome C Gene Symbol: CYCS Gene ID: 54205 Accession #: P99999 ELISA Kit: RayBio Custom Human Cytochrome C ELISA	DR6 (TNFRSF21) Gene Symbol: TNFRSF21 Gene ID: 27242 Accession #: O75509 ELISA Kit: RayBio Human DR6 ELISA	Fas (TNFRSF6/Apo-1) Gene Symbol: Fas Gene ID: 355 Accession #: P25445 ELISA Kit: RayBio Human Fas ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG Gene ID: 356 Accession #: P48023 ELISA Kit: RayBio Human FasL ELISA
HSP27 Gene Symbol: HSPB1 Gene ID: 3315 Accession #: P04792 ELISA Kit: RayBio Human HSP27 ELISA	HSP60 Gene Symbol: HSPA14 Gene ID: 51182 Accession #: Q0VDF9	HSP70 Gene Symbol: HSPA4 Gene ID: 3308 Accession #: P34932 ELISA Kit: RayBio Human HSP70 ELISA	HTRA2 Gene Symbol: HTRA2 Gene ID: 27429 Accession #: O43464 ELISA Kit: RayBio Human HTRA2 ELISA	IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019 ELISA Kit: RayBio Human IGF-1 ELISA
IGF-2 Gene Symbol: Igf2 Gene ID: 3481 Accession #: P01344	IGFBP-1 Gene Symbol: IGFBP1 Gene ID: 3484 Accession #: P08833 ELISA Kit: RayBio Human IGFBP-1 ELISA	IGFBP-2 Gene Symbol: IGFBP2 Gene ID: 3485 Accession #: P18065 ELISA Kit: RayBio Human IGFBP-2 ELISA	IGFBP-3 Gene Symbol: IGFBP3 Gene ID: 3486 Accession #: P17936 ELISA Kit: RayBio Human IGFBP-3 ELISA	IGFBP-4 Gene Symbol: IGFBP4 Gene ID: 3487 Accession #: P22692 ELISA Kit: RayBio Human IGFBP-4 ELISA
IGFBP-5 Gene Symbol: IGFBP5 Gene ID: 3488 Accession #: P24593 ELISA Kit: RayBio Human IGFBP-5 ELISA	IGFBP-6 Gene Symbol: IGFBP6 Gene ID: 3489 Accession #: P24592 ELISA Kit: RayBio Human IGFBP-6 ELISA	IGF-1 R Gene Symbol: IGF1R Gene ID: 3480 Accession #: P08069 ELISA Kit: RayBio Human IGF-1 R ELISA	livin Gene Symbol: BIRC7 Gene ID: 79444 Accession #: Q96CA5 ELISA Kit: RayBio Human Livin ELISA	p21 Gene Symbol: CDKN1A Gene ID: 1026 Accession #: P38936
p27 Gene Symbol: SSSCA1 Gene ID: 10534 Accession #: O60232 ELISA Kit: RayBio Human p27 ELISA	p53 Gene Symbol: TP53 Gene ID: 7157 Accession #: P04637 ELISA Kit: RayBio Human p53 ELISA	SMAC Gene Symbol: DIABLO Gene ID: 56616 Accession #: Q9NR28 ELISA Kit: RayBio Human SMAC ELISA	Survivin (BIRC5) Gene Symbol: BIRC5 Gene ID: 332 Accession #: O15392	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Gene ID: 7132 Accession #: P19438 ELISA Kit: RayBio Human sTNFRI ELISA
TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Gene ID: 7133 Accession #: P20333 ELISA Kit: RayBio Human sTNFRII ELISA	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta (TNFSF1B) Gene Symbol: LTA Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA	TRAIL R1 (TNFRSF10A/DR4) Gene Symbol: TNFRSF10A Gene ID: 8797 Accession #: O00220 ELISA Kit: RayBio Human TRAIL R1 ELISA	TRAIL R2 (TNFRSF10B/DR5) Gene Symbol: TNFRSF10B Gene ID: 8795 Accession #: O14763 ELISA Kit: RayBio Human TRAIL R2 ELISA
TRAIL R3 (TNFRSF10C) Gene Symbol: TNFRSF10C Gene ID: 8794 Accession #: O14798 ELISA Kit: RayBio Human TRAIL R3 ELISA	TRAIL R4 (TNFRSF10D) Gene Symbol: TNFRSF10D Gene ID: 8793 Accession #: Q9UBN6 ELISA Kit: RayBio Human TRAIL R4 ELISA	XIAP Gene Symbol: XIAP Gene ID: 331 Accession #: P98170 ELISA Kit: RayBio Human XIAP ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Apoptosis Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
Protease Inhibitor Cocktail
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Documents

Manual (AAH-APO-1.pdf)

SDS (AAH-APO-1.pdf)

Excel (AAH-APO-1.xls)

Citations

Wu B., Chu X., Feng C., et al. Heat shock protein gp96 decreases p53 stability by regulating Mdm2 E3 ligase activity in liver cancer. Cancer Lett. 2015 Apr 10;359(2):325-34. doi: 10.1016/j.canlet.2015.01.034.

Species:

Human

Sample Type:

Cell Lysate (gp96 knock down cell lines)

Spadavecchia S, Gonzalez-Lopez OD, Kyla Driscoll Carroll KD, Diana Palmeri D, Lukac DM. Convergence of KSHV Reactivation with EBV latency and cellular growth mediated by the Notch signaling pathway in co-infected cells. J Virol. 2010:84(20):10488-15000.

Species:

Human

Sample Type:

Cell Lysate (transfected EREB2-5 cell extracts)

Jackson R., Tilokee E., Latham N., et al. Paracrine Engineering of Human Cardiac Stem Cells With Insulin-Like Growth Factor 1 Enhances Myocardial Repair. J Am Heart Assoc. 2015 Sep 11;4(9):e002104. doi: 10.1161/JAHA.115.002104.

Species:

Human

Sample Type:

Cell Lysate (IGF-1 lentiviral system in explant derived stem cells.)

Jabir, M., Sahib, U., Taqi, Z., et al. Linalool-Loaded Glutathione-Modified Gold Nanoparticles Conjugated with CALNN Peptide as Apoptosis Inducer and NF-?B Translocation Inhibitor in SKOV-3 Cell Line. Int J Nanomedicine. 2020 Nov 17;15:9025-9047. doi: 10.2147/IJN.S276714. eCollection 2020.

Species:

Human

Sample Type:

Cell Lysate (SKOV-3 ovarian cancer cell lines)

Mohan S., Abdelwahab S., Kamalidehghan B., et al. Involvement of NF-?B and Bcl2/Bax signaling pathways in the apoptosis of MCF7 cells induced by a xanthone compound Pyranocycloartobiloxanthone A. Phytomedicine. 2012 Aug 15;19(11):1007-15. doi: 10.1016/j.phymed.2012.05.012.

Species:

Human

Sample Type:

Cell Lysate (Pyranocycloartobiloxanthone A apoptosis model againts breast cancer cells (MCF7))

Pastuszko P, Schears GJ, Pirzadeh A, et al. Effect of granulocyte-colony stimulating factor (G-CSF) on expression of selected proteins involved in regulation of apoptosis in the brain of newborn piglets following cardiopulmonary bypass (CPB) and deep hypothermic circulatory arrest (DHCA). The Journal of Thoracic and Cardiovascular Surgery. 2012;143(6):1436-1442. doi:10.1016/j.jtcvs.2012.01.018.

Species:

Porcine

Sample Type:

Tissue Lysate (Porcine Brain lysate)

Leslie K., Whitley G., Herse F., et al. Increased apoptosis, altered oxygen signaling, and antioxidant defenses in first-trimester pregnancies with high-resistance uterine artery blood flow. Am J Pathol. 2015 Oct;185(10):2731-41. doi: 10.1016/j.ajpath.2015.06.020.

Species:

Human

Sample Type:

Tissue Lysate (Doppler Resistence index samples placental lysates)

Zhang, D., Lee H., Cao Y., et al. miR-185 mediates lung epithelial cell death after oxidative stress. Am J Physiol Lung Cell Mol Physiol 310: L700-L710, 2016.

Species:

Human

Sample Type:

Cell Lysate (Beas2B cells under hypoxic conditions.)

Hu, Haiyan, et al. "The overexpression of hypomethylated miR-663 induces chemotherapy resistance in human breast cancer cells by targeting heparin sulfate proteoglycan 2 (HSPG2)." Journal of Biological Chemistry 288.16 (2013): 10973-10985.

Species:

Human

Sample Type:

Cell Lysate (lysates of MDA-MB-231 cells with or without miR-663)

Oo A., Rausalu K., Merits A., et al. Deciphering the potential of baicalin as an antiviral agent for Chikungunya virus infection. Antiviral Res. 2018 Feb;150:101-111. doi: 10.1016/j.antiviral.2017.12.012.

Species:

Human

Sample Type:

Cell Lysate (Human HEK 293T cells infected qith Chukungunya viral)

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