Human Apoptosis Array C1
Katalog-Nummer AAH-APO-1-2
Size : 2SampleKit
Marke : RayBiotech
Contact local distributor :
Telefonnummer : +1 850 650 7790
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Number of Targets Detected | 43 |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Research Area | Apoptosis, Akt Signaling, IGF Signaling, p53 Signaling |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| bad
| bax
| bcl-2
| bcl-w
| BID
|
| BIM
| Caspase-3
| Caspase-8
| CD40 (TNFRSF5)
| CD40 Ligand (TNFSF5)
|
| cIAP-2
| Cytochrome C
| DR6 (TNFRSF21)
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
|
| HSP27
| HSP60
| HSP70
| HTRA2
| IGF-1
|
| IGF-2
| IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
|
| IGFBP-5
| IGFBP-6
| IGF-1 R
| livin
| p21
|
| p27
| p53
| SMAC
| Survivin (BIRC5)
| TNF RI (TNFRSF1A)
|
| TNF RII (TNFRSF1B)
| TNF alpha
| TNF beta (TNFSF1B)
| TRAIL R1 (TNFRSF10A/DR4)
| TRAIL R2 (TNFRSF10B/DR5)
|
| TRAIL R3 (TNFRSF10C)
| TRAIL R4 (TNFRSF10D)
| XIAP
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Apoptosis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- Protease Inhibitor Cocktail
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Citations
Wu B., Chu X., Feng C., et al. Heat shock protein gp96 decreases p53 stability by regulating Mdm2 E3 ligase activity in liver cancer. Cancer Lett. 2015 Apr 10;359(2):325-34. doi: 10.1016/j.canlet.2015.01.034.
Species:
Human
Sample Type:
Cell Lysate (gp96 knock down cell lines)
Spadavecchia S, Gonzalez-Lopez OD, Kyla Driscoll Carroll KD, Diana Palmeri D, Lukac DM. Convergence of KSHV Reactivation with EBV latency and cellular growth mediated by the Notch signaling pathway in co-infected cells. J Virol. 2010:84(20):10488-15000.
Species:
Human
Sample Type:
Cell Lysate (transfected EREB2-5 cell extracts)
Jackson R., Tilokee E., Latham N., et al. Paracrine Engineering of Human Cardiac Stem Cells With Insulin-Like Growth Factor 1 Enhances Myocardial Repair. J Am Heart Assoc. 2015 Sep 11;4(9):e002104. doi: 10.1161/JAHA.115.002104.
Species:
Human
Sample Type:
Cell Lysate (IGF-1 lentiviral system in explant derived stem cells.)
Jabir, M., Sahib, U., Taqi, Z., et al. Linalool-Loaded Glutathione-Modified Gold Nanoparticles Conjugated with CALNN Peptide as Apoptosis Inducer and NF-?B Translocation Inhibitor in SKOV-3 Cell Line. Int J Nanomedicine. 2020 Nov 17;15:9025-9047. doi: 10.2147/IJN.S276714. eCollection 2020.
Species:
Human
Sample Type:
Cell Lysate (SKOV-3 ovarian cancer cell lines)
Mohan S., Abdelwahab S., Kamalidehghan B., et al. Involvement of NF-?B and Bcl2/Bax signaling pathways in the apoptosis of MCF7 cells induced by a xanthone compound Pyranocycloartobiloxanthone A. Phytomedicine. 2012 Aug 15;19(11):1007-15. doi: 10.1016/j.phymed.2012.05.012.
Species:
Human
Sample Type:
Cell Lysate (Pyranocycloartobiloxanthone A apoptosis model againts breast cancer cells (MCF7))
Pastuszko P, Schears GJ, Pirzadeh A, et al. Effect of granulocyte-colony stimulating factor (G-CSF) on expression of selected proteins involved in regulation of apoptosis in the brain of newborn piglets following cardiopulmonary bypass (CPB) and deep hypothermic circulatory arrest (DHCA). The Journal of Thoracic and Cardiovascular Surgery. 2012;143(6):1436-1442. doi:10.1016/j.jtcvs.2012.01.018.
Species:
Porcine
Sample Type:
Tissue Lysate (Porcine Brain lysate)
Leslie K., Whitley G., Herse F., et al. Increased apoptosis, altered oxygen signaling, and antioxidant defenses in first-trimester pregnancies with high-resistance uterine artery blood flow. Am J Pathol. 2015 Oct;185(10):2731-41. doi: 10.1016/j.ajpath.2015.06.020.
Species:
Human
Sample Type:
Tissue Lysate (Doppler Resistence index samples placental lysates)
Zhang, D., Lee H., Cao Y., et al. miR-185 mediates lung epithelial cell death after oxidative stress. Am J Physiol Lung Cell Mol Physiol 310: L700-L710, 2016.
Species:
Human
Sample Type:
Cell Lysate (Beas2B cells under hypoxic conditions.)
Hu, Haiyan, et al. "The overexpression of hypomethylated miR-663 induces chemotherapy resistance in human breast cancer cells by targeting heparin sulfate proteoglycan 2 (HSPG2)." Journal of Biological Chemistry 288.16 (2013): 10973-10985.
Species:
Human
Sample Type:
Cell Lysate (lysates of MDA-MB-231 cells with or without miR-663)
Oo A., Rausalu K., Merits A., et al. Deciphering the potential of baicalin as an antiviral agent for Chikungunya virus infection. Antiviral Res. 2018 Feb;150:101-111. doi: 10.1016/j.antiviral.2017.12.012.
Species:
Human
Sample Type:
Cell Lysate (Human HEK 293T cells infected qith Chukungunya viral)
