Cd22 Rat Monoclonal Antibody [Clone ID: 2D6]

Specifications

Product Data
Clone Name	2D6
Applications	FC
Recommended Dilution	Flow Cytometry: < / = 0.2 µg/10e6 cells. (Ref.2)
Reactivities	Mouse
Host	Rat
Isotype	IgG1
Clonality	Monoclonal
Specificity	This antibody is specific to CD22 (Mr 150 kDa), a B Lymphocyte Adhesion Molecule.
Formulation	PBS containing 0.09% Sodium Azide as preservative and a stabilizing agent. Label: APC State: Liquid purified Ig fraction. Label: Allophycocyanin
Concentration	lot specific
Conjugation	APC
Storage	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Database Link	Entrez Gene 12483 Mouse UniProt ID P35329
Background	CD22, also known as Lyb-8, is a heterodimeric transmembrane glycoprotein and a member of a structurally related group of immunoglobulin (Ig) superfamily domain-containing proteins called the sialoadhesin family. It is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface concomitant with IgD, and is found on most IgM+IgD+ mature B lymphocytes. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via cross-linking of surface Ig increases CD22 expression. (Ref.1,2) CD22 associates with the B cell receptor (BCR) complex and mediates intercellular adhesion. (Ref.2-4) Its intracellular domain is phosphorylated after antigen receptor crosslinking and is involved in negative regulation of B-cell activation. (Ref.6-9)
Synonyms	SIGLEC2, Siglec-2, B-cell receptor CD22, Leu-14, BL-CAM
Reference Data