• SKU DIA-PDL1-OD
    Specificity

    PD-L1
    Species Reactivity

    Human
    Host Species

    Rabbit
    Isotype

    IgG whole molecule
    Clone

    JAL1
    Clonality (Mono-/Polyclonal)

    monoclonal
    Application

    ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections)
    Conjugation

    unconjugated
    Dilution

    Immunohistochemistry (IHC): 1:100 – 1:400
    Format

    < 0.1% NaN3, 1% BSA, concentrate, in Tris buffer (pH 7.3-7.7), ProteinA/G purified antibody
    Product line / Topic

    Immuno-Oncology, Tumor Marker / Biology
    Intended Use

    for In vitro Diagnostic Use
    Temperature - Storage

    2-8°C
    Temperature - Transport

    cooled
    Search Code

    CD274, PD-L1, PDL1, DIAPDL1, IHC, FFPE, Paraffin
    Manufacturer / Brand

    dianova
    Uniprot_ID

    Q9NZQ7
    Gene_ID

    29126
    Alias

    B7-H, B7H1, CD274, PD-L1, PDCD1-LG1, PDCD1L1, PDCD1LG1, PDL1, Programed Death Ligand 1, Programmed cell death 1 ligand 1, Programmed Cell Death Protein 1 Ligand 1
  • Clone JAL1 has been developed specifically for routine immunohistochemical (IHC) detection of PD-L1 in formalin-fixed paraffin-embedded tissue specimen. Moreover, JAL1 has been validated for the identification of PD-L1-positive macrophages and tumor tissues under pathological conditions.

    PD-L1, also known as CD274 or B7 homolog 1 (B7-H1) is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on macrophages and activated T- and B-cells, dendritic cells, keratinocytes and monocytes.

    Moreover, several human cancer cells express PD-L1 at high levels. Binding of PD-L1 with its receptor PD-1 on T cells inhibits t cell proliferation and the production of cytokines, such as interleukin (IL)-2. It has been shown that PD-L1 helps tumor cells to evade anti-tumor immunity and blockade of PD-L1 reduces the growth of tumors in the presence of immune cells.

    PD-L1 is commonly over expressed on tumor cells or on non-transformed cells in the tumor microenvironment2. PD-L1 expressed on the tumor cells binds to PD-1 receptors on the activated T cells, which leads to the inhibition of the cytotoxic T cells. These deactivated T cells remain inhibited in the tumor microenvironment.

    PD-1 and PD-L1/PD-L2 belong to the family of immune checkpoint proteins that act as co-inhibitory factors, which can halt or limit the development of the T cell response