K2225-100 assays | Amylase Activity Colorimetric Assay Kit Biotrend

Background

Amylase is an enzyme that break starch down to sugar. Amylase is present in the saliva of humans. α-Amylase is the major form found in fungi, seeds, humans and other mammals. α-Amylase is a calcium metalloenzyme which completely unable to function in the absence of calcium. In humans, both the pancreatic and salivary amylases are major digestive enzymes. Increased amylase levels in humans are associated with salivary trauma, mumps because of renal failure, pancreatitis and inflammation of the salivary glands.

The Amylase Activity Colorimetric Assay Kit provides a sensitive, simple, fast and convenient way for detection of α-amylase activity in various samples based on colorimetric method. The assay utilizes ethylidene-pNP-G7 as the substrate. In the assay, the substrate is specifically cleaved by α-amylase to produce the smaller fragments, which can be acted upon by α-glucosidase that causes the ultimate release of the chromophore (at 405 nm). The kit can detect as low as 0.2 mU α-amylase content.

Features & Properties

Features	Fast and convenient; Simple procedure; Takes approx. less than 40 minutes; Sensitive assays for measuring amylase activity in various biological samples.
Shipping	Gel pack.
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Related Biological Data

Product Citation

1. Zamuruyev KO, Aksenov AA, et al. "Human breath metabolomics using an optimized non-invasive exhaled breath condensate sampler. J Breath Res." 2016 Dec 22;11(1):016001. PMID:28004639

Storage

Store at -20°C.

Overview

Kit components

Amylase Assay Buffer, Amylase Substrate Mix, Amylase Positive Control, Nitrophenol standard (2 mM)

Amylase Activity Colorimetric Assay Kit

Katalog-Nummer K2225-100 assays

Contact local distributor :

Marke : ApexBio Technology