Triiodothyronine ELISA Kit
Katalog-Nummer OKEH02533
Size : 96Wells
Marke : Aviva Systems Biology
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Triiodothyronine ELISA Kit (OKEH02533) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Triiodothyronine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Triiodothyronine and incubated. The Triiodothyronine found in the sample or standards competes with the biotinylated Triiodothyronine for limited binding sites on the immobilized anti-Triiodothyronine antibody. Excess unbound biotinylated Triiodothyronine and sample or standard Triiodothyronine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Triiodothyronine captured in the well and inversely proportional to the amount of Triiodothyronine which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 78-5000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 92% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 7.2% (n = 20) Mean Inter-assay CV%: < 10.1% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Target Abbr: T3 Target Name: Triiodothyronine | ||||||||||||||||||||||
:: | Pubchem: 44123465 | ||||||||||||||||||||||
:: | Chemical formula: C15H12I3NO4 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
Sensitivity | 35 pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant General Triiodothyronine | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Alias Symbols | triiodothyronine, T3, 3,3',5-triiodo-L-thyronine |
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Protein Name | Triiodothyronine |
Description of Target | The L-triiodothyronine (T3, liothyronine) thyroid hormone is normally synthesized and secreted by the thyroid gland in much smaller quantities than L-tetraiodothyronine (T4, levothyroxine, L-thyroxine). Most T3 is derived from peripheral monodeiodination of T4 at the 5 position of the outer ring of the iodothyronine nucleus. The hormone finally delivered and used by the tissues is mainly T3. |