Heparan sulfate ELISA Kit

Katalog-Nummer OKEH02552

Size : 96Wells

Marke : Aviva Systems Biology

Contact local distributor :


Telefonnummer : +1 850 650 7790
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02552
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Heparan Sulfate ELISA Kit (OKEH02552) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Heparan sulfate has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Heparan sulfate is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Heparan sulfate captured in the well.
ELISA Kit Range4.69-300ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 87%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 8.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Heparan sulfate Microplate96 Wells (12 x 8 Well strips)
Heparan sulfate Lyophilized Standard2 x 300 ng
Sample Diluent1 x 20 mL
100X Biotinylated Heparan sulfate Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: HS
Target Name: Heparan sulfate
::Pubchem: 53477714
::Chemical formula: C14H23NO21S3-2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity2.345 ng/mL
SpecificityNatural and recombinant General Heparan sulfate
Publications

Glycocalyx Preservation and NO Production in Fatty Livers-The Protective Role of High Molecular Polyethylene Glycol in Cold Ischemia Injury. Int J Mol Sci. 19, (2018). 30103565

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Alias SymbolsHS, Heparan sulfate, HS
Protein NameHeparan sulfate
Description of TargetHeparan sulfate (HS) is a linear polysaccharide found in all animal tissues. It occurs as a proteoglycan (HSPG) in which two or three HS chains are attached in close proximity to cell surface or extracellular matrix proteins. It is in this form that HS binds to a variety of protein ligands and regulates a wide variety of biological activities, including developmental processes, angiogenesis, blood coagulation and tumour metastasis. HS has been shown to serve as cellular receptor for a number of viruses including the respiratory syncytial virus.

Sie könnten auch an folgenden Produkten interessiert sein:



Katalog-Nummer
Beschreibung
Cond.
Preis zzgl. MwSt.