Folic acid ELISA Kit
Katalog-Nummer OKEH02550
Size : 96Wells
Marke : Aviva Systems Biology
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Telefonnummer : +1 850 650 7790
| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Folic acid ELISA Kit (OKEH02550) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Folic acid antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Folic acid and incubated. The Folic acid found in the sample or standards competes with the biotinylated Folic acid for limited binding sites on the immobilized anti-Folic acid antibody. Excess unbound biotinylated Folic acid and sample or standard Folic acid is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Folic acid captured in the well and inversely proportional to the amount of Folic acid which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 99% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.1% (n = 20) Mean Inter-assay CV%: < 7.3% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: FA Target Name: Folic acid | ||||||||||||||||||||||
| :: | Pubchem: 6037 | ||||||||||||||||||||||
| :: | Chemical formula: C19H19N7O6 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.12 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General Folic acid | ||||||||||||||||||||||
| Publications | Folic acid supplementation improves vascular endothelial function, yet not skin blood flow during exercise in the heat, in patients with heart failure. Am J Physiol Regul Integr Comp Physiol. 315, R810-R819 (2018). 29975566 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA | ||||||||||||||||||||||
| Description |
| Alias Symbols | FA, Folic acid, FA, Vitamin B9, Vitamin Bc, Folacin |
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| Protein Name | Folic acid |
| Description of Target | Vitamin B9 (folic acid and folate inclusive) is essential to numerous bodily functions. The human body needs folate to synthesize DNA, repair DNA, and methylate DNA as well as to act as a cofactor in biological reactions involving folate. It is especially important in aiding rapid cell division and growth, such as in infancy and pregnancy. Children and adults both require folic acid to produce healthy red blood cells and prevent anemia. Folic acid (also known as vitamin B9, vitamin Bc or folacin) and folate (the form naturally occurring in the body), as well as pteroyl-L-glutamic acid, pteroyl-L-glutamate, and pteroylmonoglutamic acid are forms of the water-soluble vitamin B9. Folic acid is itself not biologically active, but its biological importance is due to tetrahydrofolate and other derivatives after its conversion to dihydrofolic acid in the liver. |