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Mouse Monoclonal Anti-Actin, Smooth Muscle (Leiomyosarcoma Marker) [Clone 1A4; same as asm-1]
Katalog-Nummer NB-36-00687-P0
Size : 20ug
Marke : Neo Biotech
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Telefonnummer : +1 850 650 7790
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Printable Datasheet
Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody [1A4; same as asm-1]
Catalog Number Formulation Size Price
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Smooth Muscle Actin Mouse Monoclonal Antibody (1A4).
SDS-PAGE Analysis of Purified Smooth Muscle Actin Mouse Monoclonal Antibody (1A4). Confirmation of Integrity and Purity of Antibody.
Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with Smooth Muscle Actin Mouse Monoclonal Antibody (1A4) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).
Flow Cytometric Analysis of Human HeLa cells using Smooth Muscle Actin Mouse Monoclonal Antibody (1A4) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Smooth Muscle Actin Antibody in Western Blot (WB)
Western Blot Analysis of Mouse 3T3 lysate using ACTA2 Mouse Monoclonal Antibody (1A4)
Applications & Dilutions
Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol
Summary
Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Baboon, Cat, Chicken, Cow, Dog, Goat, Guinea Pig, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep
Research Areas
Cardiovascular, Muscle, Mesenchymal Stem Cell Differentiation, Signal Transduction
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cytoplasm, Cytoskeleton
Gene Name
ACTA2
Positive Control
HeLa cells, smooth muscle or leiomyosarcoma, Mouse 3T3 cells
Immunogen
N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.
Alternate Names
Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Aortic Smooth Muscle, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha 2 Actin, Alpha Actin 2, Alpha Cardiac Actin, Alpha Smooth Muscle, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46
Functions
- Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Key References
- Skalli O et. al. Journal of Cell Biology, 1986, 103:2787-96.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/2701935/
- https://pubmed.ncbi.nlm.nih.gov/2295650/
- https://pubmed.ncbi.nlm.nih.gov/14702039/
- https://pubmed.ncbi.nlm.nih.gov/15164054/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/6330528/
- https://pubmed.ncbi.nlm.nih.gov/2612915/
- https://pubmed.ncbi.nlm.nih.gov/16548883/
- https://pubmed.ncbi.nlm.nih.gov/19015515/
- https://pubmed.ncbi.nlm.nih.gov/22223895/
- https://pubmed.ncbi.nlm.nih.gov/22814378/
- https://pubmed.ncbi.nlm.nih.gov/26228148/
- https://pubmed.ncbi.nlm.nih.gov/30626964/
- https://pubmed.ncbi.nlm.nih.gov/17994018/
- https://pubmed.ncbi.nlm.nih.gov/19409525/
- https://pubmed.ncbi.nlm.nih.gov/19639654/
- https://pubmed.ncbi.nlm.nih.gov/20734336/
- https://pubmed.ncbi.nlm.nih.gov/20970362/
- https://pubmed.ncbi.nlm.nih.gov/27481187/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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