4E Binding Protein 1, phosphorylated (Thr37/46) (4EBP1, 4E-BP1, Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP) (Biotin)
Katalog-Nummer 0004-19K-100ul
Size : 100ul
Marke : US Biological
Host
rabbitConjugate
BiotinIsotype
IgGClone Number
10E1(236B4)Grade
PurifiedApplications
FC WBShipping Temp
Blue IceStorage Temp
-20°CTranslation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the eIF4E translation initiation factor. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP1to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).
Applications:
Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Flow Cytometry: 1:400.
Optimal dilutions to be determined by the researcher.
Flow Cytometry: 1:400.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Product Type
Mab
Isotype
IgG
Clone No
10E1(236B4)
Host
rabbit
Source
human
Concentration
Not Determined
Form
Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding
Thr37 and Thr46 of mouse 4E-BP1.
Specificity
Recognizes endogenous levels of human 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at analagous sites. Species crossreactivity
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.