4-1BB Ligand (4-1BBL)
Katalog-Nummer 0004-01D-500ug
Size : 500ug
Marke : US Biological
Host
ratIsotype
IgG2bClone Number
8H117Grade
Affinity PurifiedApplications
E FC WBShipping Temp
Blue IceStorage Temp
-20°C4-1BB Ligand is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF9). It is expressed on activated antigen presenting cells (APCs) and on most T and B lymphoma cell lines. Soluble 4-1BB Ligand can be released from the cell surface following cellular activation via proteolytic cleavage. 4-1BB Ligand exists as a noncovalent homotrimer and interacts with 4-1BB (TNFRSF9) to provide T cell costimulatory signals and dendritic cell activation.
Applications:
Suitable for use in Western Blot, Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilution:
Western Blot: 1-2ug/ml.
Flow Cytometry: 0.25ug/ml. 10ul of the diluted solution labels 1x10e6 cells in a total reaction volume ≤ 200ul
Optimal dilutions to be determined by the researcher.
Flow Cytometry: 0.25ug/ml. 10ul of the diluted solution labels 1x10e6 cells in a total reaction volume ≤ 200ul
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Type
Mab
Isotype
IgG2b
Clone No
8H117
Host
rat
Source
mouse
Concentration
~0.5mg/ml (after reconstitution)
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin
Immunogen
Recombinant protein corresponding to Arg104-Glu309 from mouse 4-1BB Ligand extracellular domain expressed in Sf-21.
Specificity
Recognizes mouse 4-1BB Ligand. In direct ELISA, does not crossreact with recombinant human RELT.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.