Anti-PRDX1 antibody [8E7]

Katalog-Nummer NB-22-7012-200ul

Size : 200µg

Marke : Neo Biotech

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Telefonnummer : +1 850 650 7790

  • IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
  • Western blot analysis of 1) MCF7, 2) Rat Kidney Tissue, 3) Mouse Brain Tissue, diluted at 1:2000.
  • Immunofluorescence analysis of Human-appendix tissue. 1, Peroxiredoxin 1 monoclonal antibody (8E7) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Rat-brain tissue. 1, Peroxiredoxin 1 monoclonal antibody (8E7) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, Peroxiredoxin 1 monoclonal antibody (8E7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

General Info

Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Peroxiredoxin-1 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: 8E7
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:1000-3000
IF 1:100-200
IHC 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: PRDX1
Gene ID: 5052
Uniprot ID: PRDX1_HUMAN
Specificity: The antibody detects endogenous Peroxiredoxin 1 protein.
Immunogen: Recombinant Protein of Peroxiredoxin-1

Description

Function Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
Protein Name Peroxiredoxin-1
Natural Killer Cell-Enhancing Factor A
Nkef-A
Proliferation-Associated Gene Protein
Pag
Thioredoxin Peroxidase 2
Thioredoxin-Dependent Peroxide Reductase 2
Thioredoxin-Dependent Peroxiredoxin 1
Database Links Reactome: R-HSA-3299685
Reactome: R-HSA-5628897
Reactome: R-HSA-8862803
Reactome: R-HSA-9818027
Cellular Localisation Cytoplasm
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Peroxiredoxin-1 antibody
Anti-Natural Killer Cell-Enhancing Factor A antibody
Anti-Nkef-A antibody
Anti-Proliferation-Associated Gene Protein antibody
Anti-Pag antibody
Anti-Thioredoxin Peroxidase 2 antibody
Anti-Thioredoxin-Dependent Peroxide Reductase 2 antibody
Anti-Thioredoxin-Dependent Peroxiredoxin 1 antibody
Anti-PRDX1 antibody
Anti-PAGA antibody
Anti-PAGB antibody
Anti-TDPX2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance