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> Anti-EIF4A1 antibody [M8]

Anti-EIF4A1 antibody [M8]

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Katalog-Nummer NB-22-7036

Size : 100µg

Marke : Neo Biotech

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Telefonnummer : +1 850 650 7790
Datasheet
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Product Description

Mouse Monoclonal to eIF4A1.

Product Information

Code:    NB-22-7036
ClonalityMonoclonal
HostMouse
ReactivityHuman, Mouse, Rat
ApplicationsWB, IF
ImmunogenSynthetic Peptide
ConjugationUnconjugated
GeneID1973 (Human); 13681 (Mouse);
IsotypeIgG1
DilutionrangeWB 1:1000-3000; IF 1:100-200
SpecificityThe antibody detects endogenous eIF4A1 protein
Swiss-ProtKeyP60842 (Human); P60843 (Mouse)
PurificationThe antibody was affinity-purified from mouse ascitesby affinity-chromatography using specific immunogen.
FormLiquid in PBS containing 50% glycerol, 0.5% BSA and0.02% sodium azide
ConcentrationN/A
CloneIDM8
StorageStore at -20°C. Avoid repeated freeze/thaw cycles
NoteFor research use only

  • IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
  • Western blot analysis of 1) 293T, 2) Hela, 3) HepG2, 4) Mouse Brain tissue
  • Immunofluorescence analysis of Mouse-spleen tissue. 1, eIF4A1 monoclonal antibody (M8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1, eIF4A1 monoclonal antibody (M8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, eIF4A1 monoclonal antibody (M8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 1, eIF4A1 monoclonal antibody (M8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

General Info

Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Eukaryotic initiation factor 4A-I is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: M8
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:1000-3000
IF 1:100-200
IHC 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: EIF4A1
Gene ID: 1973
Uniprot ID: IF4A1_HUMAN
Specificity: The antibody detects endogenous eIF4A1 protein.
Immunogen: Synthetic Peptide of eIF4A1

Description

Protein Name Eukaryotic Initiation Factor 4a-I
Eif-4a-I
Eif4a-I
Atp-Dependent Rna Helicase Eif4a-1
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-156827
Reactome: R-HSA-429947
Reactome: R-HSA-72649
Reactome: R-HSA-72662
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Alternative Antibody Names Anti-Eukaryotic Initiation Factor 4a-I antibody
Anti-Eif-4a-I antibody
Anti-Eif4a-I antibody
Anti-Atp-Dependent Rna Helicase Eif4a-1 antibody
Anti-EIF4A1 antibody
Anti-DDX2A antibody
Anti-EIF4A antibody

Information sourced from Uniprot.org

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