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> Anti-MME antibody [5B8]

Anti-MME antibody [5B8]

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Katalog-Nummer NB-22-6972-200ul

Size : 200µg

Marke : Neo Biotech

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Telefonnummer : +1 850 650 7790

  • Immunohistochemical analysis of paraffin-embedded human-breast-cancer using antibody diluted at 1:50.
  • Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, CD10 monoclonal antibody (5B8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, CD10 monoclonal antibody (5B8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, CD10 monoclonal antibody (5B8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of rat-kidney tissue. 1, CD10 monoclonal antibody (5B8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-uterus tissue. 1, CD10 monoclonal antibody (5B8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

General Info

Host: Mouse
Applications: IF/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Neprilysin is suitable for use in Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 5B8
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IF 1:50-200
WB 500-2000
1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: MME
Gene ID: 4311
Uniprot ID: NEP_HUMAN
Specificity: The antibody detects endogenous CD10 proteins.
Immunogen: Synthetic Peptide of CD10

Description

Function Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Protein Name Neprilysin
Atriopeptidase
Common Acute Lymphocytic Leukemia Antigen
Calla
Enkephalinase
Neutral Endopeptidase 24.11
Nep
Neutral Endopeptidase
Skin Fibroblast Elastase
Sfe
Cd Antigen Cd10
Database Links Reactome: R-HSA-2022377
Reactome: R-HSA-5578768
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Neprilysin antibody
Anti-Atriopeptidase antibody
Anti-Common Acute Lymphocytic Leukemia Antigen antibody
Anti-Calla antibody
Anti-Enkephalinase antibody
Anti-Neutral Endopeptidase 24.11 antibody
Anti-Nep antibody
Anti-Neutral Endopeptidase antibody
Anti-Skin Fibroblast Elastase antibody
Anti-Sfe antibody
Anti-Cd Antigen Cd10 antibody
Anti-MME antibody
Anti-EPN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance