Anti-Phospho-EIF4G1-Ser1148 antibody (1050-1130)

Katalog-Nummer NB-22-1130-200ul

Size : 200µg

Marke : Neo Biotech

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Telefonnummer : +1 850 650 7790

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eIF4G (Phospho-Ser1108) Antibody. The picture on the right is blocked with the phospho peptide.
  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eIF4G (Phospho-Ser1108) Antibody

General Info

Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Eukaryotic translation initiation factor 4 gamma 1-Ser1148 (1074-1123 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: EIF4G1
Gene ID: 1981
Uniprot ID: IF4G1_HUMAN
Immunogen Region: 1074-1123 aa
Specificity: Phospho-eIF4G (S1148) Polyclonal Antibody detects endogenous levels of eIF4G protein only when phosphorylated at S1148.
Immunogen: The antiserum was produced against synthesized peptide derived from the human eIF4G around the phosphorylation site of Ser1108 at the amino acid range 1074-1123

Description

Function Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon. Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning. As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation.
Protein Name Eukaryotic Translation Initiation Factor 4 Gamma 1
Eif-4-Gamma 1
Eif-4g 1
Eif-4g1
P220
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-156827
Reactome: R-HSA-166208
Reactome: R-HSA-429947
Reactome: R-HSA-450408
Reactome: R-HSA-72649
Reactome: R-HSA-72662
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Reactome: R-HSA-9010553
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Nucleus
Stress Granule
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 4 Gamma 1 antibody
Anti-Eif-4-Gamma 1 antibody
Anti-Eif-4g 1 antibody
Anti-Eif-4g1 antibody
Anti-P220 antibody
Anti-EIF4G1 antibody
Anti-EIF4F antibody
Anti-EIF4G antibody
Anti-EIF4GI antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance