TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) BioAssay™ ELISA Kit (Mouse)

Serum, Plasma, Biological Fluids

This BioAssay™ kit is a sandwich ELISA for in vitro quantitative measurement of TIMP-3 in Mouse serum, plasma and other biological fluids

0.094ng/mL

0.156-10ng/mL

Mouse

This BioAssay™ ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to mouse TIMP-3 (Tissue Inhibitors of Metalloproteinase 3). Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for mouse TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain mouse TIMP-3 (Tissue Inhibitors of Metalloproteinase 3), biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of mouse TIMP-3 (Tissue Inhibitors of Metalloproteinase 3). The concentration of mouse TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) in samples can be calculated by comparing the OD of the samples to the standard curve.

Microtiter Strips, 8x12 wells
Standard, 2 vials
Sample/Standard Diluent Buffer, 1x20ml
Antibody-Biotin, 1x120ul
Antibody-Biotin Diluent, 1x10ml
HRP Conjugate, 1x120ul
HRP Conjugate Diluent, 1x10ml
Substrate Reagent, 1x10ml
Stop Solution, 1x10ml
Wash Buffer (25X), 1x30ml

Unused kit is stable for 6 months after receipt.  Store Microtiter Strips, Standard, and Antibody-Biotin at 4°C if kit will be used within 30 days; otherwise store at -20°C until expiration date. Store the rest of the components at 4°C. Protect HRP Conjugate and Substrate Reagent from light. For maximum recovery, centrifuge the original vials after thawing and prior to removing the cap.

Mouse

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.