ADAM 10, CT (A Disintegrin And Metalloproteinase 10, MADM, Kuzbanian)

Proinflammatory cytokine tumor necrosis factor-a (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta. The genes encoding human, mouse and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Western Blot: 1:500-1:2000
Immunohistochemistry: 2ug/ml
Optimal dilution to be determined by the researcher.

Jurkat whole cell lysate (85kD band).

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Pab

IgG

rabbit

human

~1mg/ml

Supplied as a liquid in PBS, 0.02% sodium azide.

Purified by ion-exchange chromatography.

Synthetic peptide corresponding to aa732-748 of human ADAM10.

Recognizes human ADAM10 (CT). Species Sequence Homology

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.