Proteinase K / PROK Rabbit Polyclonal Antibody

CAT#: AP21385AF-N

Proteinase K / PROK rabbit polyclonal antibody, Azide Free


Product Images

Specifications

Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommended Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer's own choice.
Recommended Working Dilutions: vary widely, but may be between 1/10000 and 1/1000000. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day.
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Proteinase K isolated and purified from Tritirachium album. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity IgG (7S) fraction of polyclonal Rabbit antiserum to Proteinase K from Tritirachium album.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Formulation PBS, pH 7.2 without preservatives and foreign proteins
State: Azide Free
State: Lyophilized Hyperimmune purified IgG fraction
Reconstitution Method Restore by adding 1.0 ml of sterile distilled water
Concentration lot specific
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation Unconjugated
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Stability Shelf life: one year from despatch.

Synonyms Endopeptidase K, Tritirachium alkaline proteinase
Reference Data

Documents