Matrix Metalloproteinase 9 (MMP9) [KO Validated]
Katalog-Nummer 502725-200ul
Size : 200ul
Marke : US Biological
Host
mouseIsotype
IgG2b,kClone Number
16Grade
Affinity PurifiedApplications
E IC IHC IP WB KOShipping Temp
Blue IceStorage Temp
-20°CProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Applications:
Suitable for use in ELISA, Immunoprecipitation, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 0.5-5ug/ml
Immunohistochemistry (frozen/paraffin): 5-30ug/ml
Immunocytochemistry: 5-30ug/ml
ELISA: 0.05-2ug/ml
Optimal dilutions to be determined by the researcher.
Immunohistochemistry (frozen/paraffin): 5-30ug/ml
Immunocytochemistry: 5-30ug/ml
ELISA: 0.05-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Product Type
Mab
Isotype
IgG2b,k
Clone No
16
Host
mouse
Source
human
Concentration
~1mg/ml
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Purity
Purified by Protein A/G affinity chromatography.
Immunogen
Recombinant protein corresponding to Gly213-Ala399 from human MMP9, expressed in E. coli.
Specificity
Recognizes human MMP9. Species Crossreactivity
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.