Matrix Metalloproteinase 9 (MMP9) [KO Validated]

Katalog-Nummer 502725-200ul

Size : 200ul

Marke : US Biological

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Telefonnummer : +1 850 650 7790


Host
mouse

Isotype
IgG2b,k

Clone Number
16

Grade
Affinity Purified

Applications
E IC IHC IP WB KO

Shipping Temp
Blue Ice

Storage Temp
-20°C

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Applications:
Suitable for use in ELISA, Immunoprecipitation, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5-5ug/ml
Immunohistochemistry (frozen/paraffin): 5-30ug/ml
Immunocytochemistry: 5-30ug/ml
ELISA: 0.05-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Type
Mab

Isotype
IgG2b,k

Clone No
16

Host
mouse

Source
human

Concentration
~1mg/ml

Form
Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.

Purity
Purified by Protein A/G affinity chromatography.

Immunogen
Recombinant protein corresponding to Gly213-Ala399 from human MMP9, expressed in E. coli.

Specificity
Recognizes human MMP9. Species Crossreactivity


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.