Matrix Metalloproteinase 13 (MMP13) High Sensitivity BioAssay™ ELISA Kit (Mouse)

The Matrix Metalloproteinase 13 (MMP13) High Sensitivity BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MMP13 in mouse serum, plasma, tissue homogenates and other biological fluids.

15.6-1000pg/ml

6.3pg/ml

CV<10%

CV<12%

*517426A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use.
*517426B: Standard, 2x1vial
517426C: Standard Diluent, 1x20ml
*517426D: Detection Reagent A (green), 1x120ul
*517426E: Detection Reagent B (red), 1x120ul
517426F: Assay Diluent A, 1x12ml
517426G: Assay Diluent B, 1x12ml
517426H: TMB Substrate, 1x9ml
*517426J: Positive Control, 196pg/ml, 1x1vial (Lyophilized)
517426K: Stop Solution, 1x6ml
517426L: Wash Buffer, 30X, 1x20ml

The Stop Solution (517426K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Store *517426A, *517426B, *517426D, *517426E and *517426J at -20°C. After reconstitution of *5174268, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 517426J: Positive Control

The positive control is from a mouse serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

The positive control vial contains mouse MMP13 in mouse serum (lyophilized powder). Note: The mouse based serum used in this product are from healthy mouse of SPF standard (Specific Pathogen-Free animals).

196pg/ml

Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.