Matrix Metalloproteinase 13 (MMP13) High Sensitivity BioAssay™ ELISA Kit (Human)

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MMP13 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

12.1pg/ml

31.2-2000pg/ml

This assay has high sensitivity and excellent specificity for detection of High Sensitive Matrix Metalloproteinase 13 (MMP13). No significant cross-reactivity or interference between High Sensitive Matrix Metalloproteinase 13 (MMP13) and analogues was observed.

Intra-Assay: CV<10%
Inter-Assay: CV<12%

The microplate provided in this kit has been pre-coated with an antibody specific to MMP13. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to MMP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MMP13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96-well strip plate, 1x Plate
Standard, 2x vial
Detection Reagent A, 1×120ul
Detection Reagent B, 1×120ul
TMB Substrate, 1×9ml
Wash Buffer (30X), 1×20ml
Standard Diluent, 1×20ml
Assay Diluent A, 1×12ml
Assay Diluent B, 1×12ml
Stop Solution, 1×6ml

Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.