Matrix Metalloproteinase 10 (MMP10) BioAssay™ ELISA Kit (Rabbit)

Serum, plasma, tissue homogenates and other biological fluids

The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MMP10 in rabbit serum, plasma, tissue homogenates and other biological fluids.

25.2pg/ml

62.50-4000pg/ml

This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 10 (MMP10). No significant cross-reactivity or interference between Matrix Metalloproteinase 10 (MMP10) and analogues was observed.

Intra-Assay: CV<10%
Inter-Assay: CV<12%

The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MMP10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP10, biotin-conjugated
antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at
a wavelength of 450nm ± 10nm. The concentration of MMP10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96-well strip plate, 1x Plate
Standard, 2x vial
Detection Reagent A, 1×120ul
Detection Reagent B, 1×120ul
TMB Substrate, 1×9ml
Wash Buffer (30X), 1×20ml
Standard Diluent, 1×20ml
Assay Diluent A, 1×12ml
Assay Diluent B, 1×12ml
Stop Solution, 1×6ml

Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.