IPTG [367-93-1]
Katalog-Nummer HY-15921-1ml
Size : 10mM/1mL
Marke : MedChemExpress
IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.
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IPTG Chemische Struktur
CAS. Nr. : 367-93-1
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- Screening of thymidylate synthase (TYMS) overexpression conditions.
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IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. | |
In Vitro | IPTG uptake by E. coli can be independent of the action of lactose permease, since other transport pathways are also involved. At low concentration, IPTG enters cells through lactose permease, but at high concentrations (typically used for protein induction), IPTG can enter the cells independently of lactose permease. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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[1]. Isopropyl β-D-1-thiogalactopyranoside
* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO / H2O | 1 mM | 4.1964 mL | 20.9820 mL | 41.9639 mL | 104.9098 mL |
5 mM | 0.8393 mL | 4.1964 mL | 8.3928 mL | 20.9820 mL | |
10 mM | 0.4196 mL | 2.0982 mL | 4.1964 mL | 10.4910 mL | |
15 mM | 0.2798 mL | 1.3988 mL | 2.7976 mL | 6.9940 mL | |
20 mM | 0.2098 mL | 1.0491 mL | 2.0982 mL | 5.2455 mL | |
25 mM | 0.1679 mL | 0.8393 mL | 1.6786 mL | 4.1964 mL | |
30 mM | 0.1399 mL | 0.6994 mL | 1.3988 mL | 3.4970 mL | |
40 mM | 0.1049 mL | 0.5245 mL | 1.0491 mL | 2.6227 mL | |
50 mM | 0.0839 mL | 0.4196 mL | 0.8393 mL | 2.0982 mL | |
60 mM | 0.0699 mL | 0.3497 mL | 0.6994 mL | 1.7485 mL | |
80 mM | 0.0525 mL | 0.2623 mL | 0.5245 mL | 1.3114 mL | |
100 mM | 0.0420 mL | 0.2098 mL | 0.4196 mL | 1.0491 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
IPTG367-93-1Isopropyl β-D-thiogalactosideBiochemical Assay ReagentsInhibitorinhibitorinhibit
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- Produktname:
- IPTG
- Art. -Nr.:
- HY-15921
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