Narcissus pseudonarcissus Lectin (NPA, NPL)
Katalog-Nummer N0204-10-5mg
Size : 5mg
Marke : US Biological
Grade
Molecular Biology GradeShipping Temp
Blue IceStorage Temp
-20°CDaffodil
Daffodil lectin exists as a dimeric protein composed of two subunits of 13,000D devoid of attached carbohydrate. Below pH 5.0 and above pH 9.0 NPL dissociates into monomers. This lectin has a specificity toward alpha linked mannose, preferring polymannose structures containing (a-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA and PSA, NPL does not bind glucose. Staining of the cytoplasmic membrane of cells with NPL after short-term acid treatment can indicate those cells undergoing apoptosis. Detection of apoptotic cells using NPL binding is a highly sensitive and reliable tool for studying cell death.
Applications:
User-determined
Inhibiting Sugar:
100mM mannose
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Daffodil bulbs
Purity
Homogeneous by SDS-PAGE
Concentration
Not determined
Form
Supplied as a lyophilized powder.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.