| |
Product Description
Human Primary Dermal Fibroblasts from Cell Biologics are isolated from normal adult human skin tissue. Human Primary Dermal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml.
Product Testing
Human Primary Dermal Fibroblasts from Cell Biologics are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Human Primary Dermal Fibroblasts are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Laboratory Applications
Human Primary Dermal Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Storage of Cell Biologics’ Products
Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C.
Authorized Uses of Cell Biologics’ Products
Human Primary Dermal Fibroblasts from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any cells or products from the purchaser to other markets, organizations or individuals is prohibited by without the company’s written consent. Terms and Conditions must be accepted before submitting an order.
Disclaimer
Appropriate safety procedures should always be used with this material. Although Human Primary Dermal Fibroblasts are isolated from normal human tissue, investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.