BCIP, Na[102185-33-1]

BCIP (5-Bromo-4-chloro-3-indoxyl phosphate) is the most widely used chromogenic phosphatase substrate, which forms a dark blue (λ_max 615 nm) precipitate on enzymatic hydrolysis. It is often used with the oxidant NBT (nitro blue tetrazolium chloride, 10008), which facilitates the precipitation, to detect alkaline phosphatase-activity and -labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization.

• Colormetric detection of alkaline phosphatase activity and labels
• Compatible with a variety of  applications
• Use alone or in combination with NBT
• White solid soluble in water

For your convenience, we offer a  BCIP/NBT Kit (10003)  that contains both reagents. We also offer Alkaline Phosphatase Conjugated Antibodies.

Find the Right Stain for your Application

The original BCIP forms a dark blue (λ_max 615 nm) precipitate and is available in two different salt formulations; BCIP, toluidine salt is soluble in DMF while BCIP, sodium salt is soluble in water. We also offer a Pink BCIP derivative, which produces a pink colored (λ_max 540 nm) precipitate. BCIP Red produces a red colored (λ_max 565 nm) precipitate. Please see our BCIP Kits that are paired with NBT (nitro blue tetrazolium chloride) for user convenience.

Molecular Structure: