IGF-1 BioAssay™ ELISA Kit (Chinese Hamster) (Insulin-like Growth Factor 1)

Insulin-like growth factor 1(IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Chinese hamster IGF1 is a single chain 70aa polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6kD. The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4 encode the 195aa precursor(IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide(IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins(IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

Cell culture supernates, serum, plasma (heparin or EDTA).

Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Chinese Hamster IGF-1.

<5pg/ml

62.5pg/ml-4000pg/ml

Recognizes natural and recombinant Chinese Hamster IGF-1. There is detectable cross-reactivity with IGF-2 (< 1%).

This Chinese Hamster IGF-1 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Chinese Hamster IGF-1 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: G49-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-1 specific for Chinese Hamster IGF-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Chinese Hamster IGF-1 amount of sample captured in plate.

96-well plate precoated with anti-Chinese Hamster IGF-1 antibody, 1x plate
Lyophilized recombinant Chinese Hamster IGF-1 standard, 2x10ng/tube
Biotinylated anti-Chinese Hamster IGF-1 antibody, 1x130ul (dilution 1:100)
Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)
Sample diluent buffer, 1x30ml
Antibody diluent buffer, 1x12ml
ABC diluent buffer, 1x12ml
TMB color developing agent, 1x10ml
TMB stop solution, 1x10ml
PBS washing buffer Powder for 1000ml, 1x buffer

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards