IFNG ELISA Kit (Hamster )
Katalog-Nummer OKWB00238
Size : 96Wells
Marke : Aviva Systems Biology
Contact local distributor :
Telefonnummer : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for IFNG ELISA Kit (Hamster) (OKWB00238) |
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| Predicted Species Reactivity | Hamster|Mesocricetus auratus | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | Aviva Systems Biology Ifng ELISA Kit (Hamster ) (OKWB00238) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ifng has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ifng is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ifng captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 15.625-1000pg/ml | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Mean Intra-Assay Precision <10% Mean Inter-Assay Precision <12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 9.4 pg/mL | ||||||||||||||||||||||
| Assay Info | Assay Type: Quantitative Sandwich ELISA |
| Gene Symbol | Ifng |
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| Gene Full Name | interferon gamma |
| Alias Symbols | IFN-gamma;interferon gamma. |
| NCBI Gene Id | 101825431 |
| Protein Name | Interferon gamma |
| Description of Target | Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons (By similarity). |
| Uniprot ID | O35497 |
| Protein Accession # | NP_001268560 |
| Nucleotide Accession # | NM_001281631 |