Vanillylmandelic acid ELISA Kit
Katalog-Nummer OKEH02588
Size : 96Wells
Marke : Aviva Systems Biology
Contact local distributor :
Telefonnummer : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Vanillylmandelic acid ELISA Kit (OKEH02588) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Vanillylmandelic acid antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Vanillylmandelic acid and incubated. The Vanillylmandelic acid found in the sample or standards competes with the biotinylated Vanillylmandelic acid for limited binding sites on the immobilized anti-Vanillylmandelic acid antibody. Excess unbound biotinylated Vanillylmandelic acid and sample or standard Vanillylmandelic acid is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Vanillylmandelic acid captured in the well and inversely proportional to the amount of Vanillylmandelic acid which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 7.8-500ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 109% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 5.2% (n = 20) Mean Inter-assay CV%: < 8.6% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: VMA Target Name: Vanillylmandelic acid | ||||||||||||||||||||||
| :: | Pubchem: 1245 | ||||||||||||||||||||||
| :: | Chemical formula: C9H10O5 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 2.3 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General Vanillylmandelic acid | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Alias Symbols | VMA, Vanillylmandelic acid, VMA |
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| Protein Name | Vanillylmandelic acid |
| Description of Target | Vanillyl mandelic acid (VMA) is an end-stage metabolite of the catecholamines epinephrine and norepinephrine. It is produced via intermediary metabolites. VMA is found in the urine, along with other catecholamine metabolites, including homovanillic acid (HVA), metanephrine and normetanephrine. In timed urine tests the quantity excreted (usually per 24 hours) is assessed, along with creatinine clearance, and the quantity of cortisols, catecholamines, and metanephrines excreted. |