Trichothecenes ELISA Kit

Katalog-Nummer OKAO00143

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for Trichothecenes ELISA Kit (OKAO00143)
Product Info
Predicted Species ReactivityGeneral
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology Trichothecenes ELISA Kit (OKAO00143) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Trichothecenes has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Trichothecenes antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
ELISA Kit Range0.2-16.2 ppb
ELISA Kit Recovery
SampleAmount
Peanut, Corn110% +/- 15%
Oats, Soybean, Feed105% +/- 15%
Wheat110% +/- 15%
ELISA Kit ReproducibilityMean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
ELISA Kit Component
ComponentAmount
Trichothecenes Microplate96 Wells (12 x 8 Well Strips)
Trichothecenes Standards6 x 1 mL
Trichothecenes Rabbit Detector Antibody1 x 6 mL
Anti-Rabbit / HRP Conjugate1 x 6 mL
Detection Reagent A1 x 6 mL
Detection Reagent B1 x 6 mL
Stop Solution1 x 6 mL
20X Wash Buffer2 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypePeanut, corn, oats, soybean, feed, wheat
Sensitivity
SampleAmount
Peanut, Corn24 ppb
Oats, Soybean, Feed24 ppb
Wheat20 ppb
Assay InfoAssay Methodology: Competitive Inhibition ELISA
Alias SymbolsT-2
Protein NameTrichothecenes
Description of TargetTrichothecenes are a very large family of chemically related mycotoxins produced by various species of Fusarium, Myrothecium, Trichoderma, Trichothecium, Cephalosporium, Verticimonosporium, and Stachybotrys. Trichothecenes belong to sesquiterpene compounds. The most important structural features causing the biological activities of trichothecenes are: the 12,13-epoxy ring, the presence of hydroxyl or acetyl groups at appropriate positions on the trichothecene nucleus and the structure and position of the side-chain. They are produced on many different grains like wheat, oats or maize by various Fusarium species such as F. graminearum, F. sporotrichioides, F. poae and F. equiseti.