Trichothecenes ELISA Kit
Katalog-Nummer OKAO00143
Size : 96Wells
Marke : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for Trichothecenes ELISA Kit (OKAO00143) |
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Predicted Species Reactivity | General | ||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||
ELISA Kit Duration | ~4 Hours | ||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Trichothecenes ELISA Kit (OKAO00143) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Trichothecenes has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Trichothecenes antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample. | ||||||||||||||||||
ELISA Kit Range | 0.2-16.2 ppb | ||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Mean Intra-Assay: CV<8% (n= 3 x 20) Mean Inter-Assay: CV<12% (n= 3 x 40) | ||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||
Sample Type | Peanut, corn, oats, soybean, feed, wheat | ||||||||||||||||||
Sensitivity |
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Assay Info | Assay Methodology: Competitive Inhibition ELISA |
Alias Symbols | T-2 |
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Protein Name | Trichothecenes |
Description of Target | Trichothecenes are a very large family of chemically related mycotoxins produced by various species of Fusarium, Myrothecium, Trichoderma, Trichothecium, Cephalosporium, Verticimonosporium, and Stachybotrys. Trichothecenes belong to sesquiterpene compounds. The most important structural features causing the biological activities of trichothecenes are: the 12,13-epoxy ring, the presence of hydroxyl or acetyl groups at appropriate positions on the trichothecene nucleus and the structure and position of the side-chain. They are produced on many different grains like wheat, oats or maize by various Fusarium species such as F. graminearum, F. sporotrichioides, F. poae and F. equiseti. |