Thromboxane A2 ELISA Kit

Katalog-Nummer OKEH02590

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Thromboxane A2 ELISA Kit (OKEH02590) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Thromboxane A2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Thromboxane A2 and incubated. The Thromboxane A2 found in the sample or standards competes with the biotinylated Thromboxane A2 for limited binding sites on the immobilized anti-Thromboxane A2 antibody. Excess unbound biotinylated Thromboxane A2 and sample or standard Thromboxane A2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Thromboxane A2 captured in the well and inversely proportional to the amount of Thromboxane A2 which was contained in the sample or standard.
ELISA Kit Range3.12-200pg/mL
ELISA Kit Recovery<= 6.6%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 6.6% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Thromboxane A2 Microplate96 Wells (12 x 8 Well strips)
Thromboxane A2 Lyophilized Standard2 x 200 pg
Sample Diluent1 x 20 mL
100X Thromboxane A2-Biotin Complex1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: TXA2
Target Name: Thromboxane A2
::Pubchem: 5280497
::Chemical formula: C20H32O5
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity1.25 pg/mL
SpecificityNatural and recombinant General Thromboxane A2
Publications

Targeted inhibition of thrombin attenuates murine neonatal necrotizing enterocolitis. Proc Natl Acad Sci U S A. 117, 10958-10969 (2020). 32366656

Assay InfoAssay Methodology: Quantitative Competitive ELISA
Description
Alias SymbolsTXA2, Thromboxane A2, TXA2
Protein NameThromboxane A2
Description of TargetThromboxane A2 (TXA2) is a thromboxane. It is produced by activated platelets and has prothrombotic properties: it stimulates activation of new platelets as well as increases platelet aggregation. This is achieved by mediating expression of the glycoprotein complex GP IIb/IIIa in the cell membrane of platelets. Circulating fibrinogen binds these receptors on adjacent platelets, further strengthening the clot. TXA2 is generated from prostaglandin H2 by thromboxane-A synthase. Aspirin irreversibly inhibits platelet cyclooxygenase 1 preventing the formation of prostaglandin H2, and therefore thromboxane A2.