Thromboxane A2 ELISA Kit
Katalog-Nummer OKEH02590
Size : 96Wells
Marke : Aviva Systems Biology
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Thromboxane A2 ELISA Kit (OKEH02590) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Thromboxane A2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Thromboxane A2 and incubated. The Thromboxane A2 found in the sample or standards competes with the biotinylated Thromboxane A2 for limited binding sites on the immobilized anti-Thromboxane A2 antibody. Excess unbound biotinylated Thromboxane A2 and sample or standard Thromboxane A2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Thromboxane A2 captured in the well and inversely proportional to the amount of Thromboxane A2 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 3.12-200pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery | <= 6.6% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.3% (n = 20) Mean Inter-assay CV%: < 6.6% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Target Abbr: TXA2 Target Name: Thromboxane A2 | ||||||||||||||||||||||
:: | Pubchem: 5280497 | ||||||||||||||||||||||
:: | Chemical formula: C20H32O5 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
Sensitivity | 1.25 pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant General Thromboxane A2 | ||||||||||||||||||||||
Publications | Targeted inhibition of thrombin attenuates murine neonatal necrotizing enterocolitis. Proc Natl Acad Sci U S A. 117, 10958-10969 (2020). 32366656 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA | ||||||||||||||||||||||
Description |
Alias Symbols | TXA2, Thromboxane A2, TXA2 |
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Protein Name | Thromboxane A2 |
Description of Target | Thromboxane A2 (TXA2) is a thromboxane. It is produced by activated platelets and has prothrombotic properties: it stimulates activation of new platelets as well as increases platelet aggregation. This is achieved by mediating expression of the glycoprotein complex GP IIb/IIIa in the cell membrane of platelets. Circulating fibrinogen binds these receptors on adjacent platelets, further strengthening the clot. TXA2 is generated from prostaglandin H2 by thromboxane-A synthase. Aspirin irreversibly inhibits platelet cyclooxygenase 1 preventing the formation of prostaglandin H2, and therefore thromboxane A2. |