Taurocholic Acid ELISA Kit
Katalog-Nummer OKCD02290
Size : 96Wells
Marke : Aviva Systems Biology
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| Datasheets/Manuals | Printable datasheet for Taurocholic Acid ELISA Kit (OKCD02290) |
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| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 2h | ||||||||||||||||||||||
| ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Taurocholic Acid (TCA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Taurocholic Acid (TCA) and unlabeled Taurocholic Acid (TCA) (Standards or samples) with the pre-coated antibody specific to Taurocholic Acid (TCA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Taurocholic Acid (TCA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Taurocholic Acid (TCA) in the sample. | ||||||||||||||||||||||
| ELISA Kit Range | 3.70-300ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Taurocholic Acid (TCA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Taurocholic Acid (TCA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | CAS: 81-24-3 PubChem: 6675 | ||||||||||||||||||||||
| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Biological agents | ||||||||||||||||||||||
| Sensitivity | 1.41 ng/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Competitive Inhibition Immunoassay |
| Gene Symbol | TCA |
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| Alias Symbols | TAUROCHOLIC ACID; Taurocholate; Cholaic acid; Cholyltaurine; Cholic acid taurine conjugate |
| Protein Name | Taurocholic Acid |
| Description of Target | TAUROCHOLIC ACID is the product of conjugation of cholic acid with taurine. Its sodium salt is the chief ingredient of the bile of carnivorous animals. It acts as a detergent to solubilize fats for absorption and is itself absorbed. It is used as a cholagogue and cholerectic. |