Sulfathiazole ELISA Kit

Katalog-Nummer OKAO00107

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for Sulfathiazole ELISA Kit (OKAO00107)
Product Info
Predicted Species ReactivityGeneral
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology Sulfathiazole ELISA Kit (OKAO00107) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Sulfathiazole has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Sulfathiazole antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
ELISA Kit Range0.5-40.5 ppb
ELISA Kit Recovery
SampleAmount
Tissue85% +/- 10%
Honey95% +/- 10%
ELISA Kit ReproducibilityMean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
ELISA Kit Component
ComponentAmount
Sulfathiazole Microplate96 Wells (12 x 8 Well Strips)
Sulfathiazole Standards6 x 1 mL
Sulfathiazole Rabbit Detector Antibody1 x 7 mL
Anti-Rabbit / HRP Conjugate1 x 7 mL
Detection Reagent A1 x 7 mL
Detection Reagent B1 x 7 mL
Stop Solution1 x 7 mL
20X Wash Buffer2 x 20 mL
2X Sample Prep Buffer2 x 20 mL
Additional InformationCAS: 72-14-0
PubChem: 5340
::Molecular Formula: C9H9N3O2S2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue (meat, chicken, duck, liver, shrimp, fish, egg), Serum, Honey, Milk.
Sensitivity
SampleAmount
Tissue1 ppb
Honey1 ppb
Specificity
SubstanceCross Reactivity Rate
Sulfathiazole100%
Sulfadimethoxypyrimidine10%
Sulfamerazine12%
Sulfadiazine4%
Sulfamethoxazole1%
Assay InfoAssay Methodology: Competitive Inhibition ELISA
Alias SymbolsST, Sulfathiazole, Sulphathiazole, 72-14-0, Sulfanilamidothiazole, 2-Sulfanilamidothiazole, 2-Sulfonamidothiazole
Protein NameSulfathiazole
Description of TargetSulfathiazole is a short-acting sulfonamide antibiotic. Its use has been largely replaced with less toxic alternatives but is still used in combination with sulfacetamide and sulfabenzamide for the treatment of vaginal infections and for disinfecting home aquariums.
Molecular Weight255.31666 g/mol