Sulfamethoxazole ELISA Kit
Katalog-Nummer OKAO00108
Size : 96Wells
Marke : Aviva Systems Biology
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| Datasheets/Manuals | Printable datasheet for Sulfamethoxazole ELISA Kit (OKAO00108) |
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| Predicted Species Reactivity | General | ||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||
| ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Sulfamethoxazole ELISA Kit (OKAO00108) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Sulfamethoxazole has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Sulfamethoxazole antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample. | ||||||||||||||||||||
| ELISA Kit Range | 0.1-8.1 ppb | ||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Mean Intra-Assay: CV<8% (n= 3 x 20) Mean Inter-Assay: CV<12% (n= 3 x 40) | ||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | CAS: 723-46-6 PubChem: 5329 | ||||||||||||||||||||
| :: | Molecular Formula: C10H11N3O3S | ||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||
| Sample Type | Tissue (meat, chicken, duck, liver, shrimp, fish), serum, urine, egg, honey, milk, feed | ||||||||||||||||||||
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| Assay Info | Assay Methodology: Competitive Inhibition ELISA |
| Alias Symbols | SMZ, Sulfamethoxazole, 723-46-6, Sulphamethoxazole, Gantanol, Sulfisomezole, Sulfamethoxazol |
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| Protein Name | Sulfamethoxazole |
| Description of Target | Sulfamethoxazole is a sulfonamide bacteriostatic antibiotic that is most commonly used in combination with trimethoprim as the drug Bactrim. Sulfamethoxazole competitively inhibits dihydropteroate synthase preventing the formation of dihydropteroic acid, a precursor of folic acid which is required for bacterial growth. |
| Molecular Weight | 253.27764 g/mol |