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> Semicarbazide hydrochloride ELISA Kit

Semicarbazide hydrochloride ELISA Kit

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Katalog-Nummer OKAO00100

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for Semicarbazide hydrochloride ELISA Kit (OKAO00100)
Product Info
Predicted Species ReactivityGeneral
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology Semicarbazide hydrochloride ELISA Kit (OKAO00100) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Semicarbazide hydrochloride has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Semicarbazide hydrochloride antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
ELISA Kit Range0.05-4.05 ppb
ELISA Kit Recovery
SampleAmount
Tissue, Liver90% +/- 15%
Honey, milk80% +/- 15%
Intestine80% +/- 15%
ELISA Kit ReproducibilityMean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
ELISA Kit Component
ComponentAmount
Semicarbazide hydrochloride Microplate96 Wells (12 x 8 Well Strips)
Semicarbazide hydrochloride Standards (81, 27, 9, 3, 1, 0 ppb)6 x 1 mL
Semicarbazide hydrochloride Rabbit Detector Antibody1 x 7 mL
Anti-Rabbit / HRP Conjugate1 x 7 mL
Detection Reagent A1 x 7 mL
Detection Reagent B1 x 7 mL
2X Sample Prep Buffer2 x 20 mL
20X Wash Buffer2 x 20 mL
2-Nitrobenzaldehyde (C7H5NO3)1 x 10 mL
Stop Solution1 x 7 mL
Additional InformationCAS: 563-41-7
PubChem: 11236
::Molecular Formula: CH6ClN3O
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue (Chicken, duck, porcine meat, beef and mutton, fish, shrimp), liver (Chicken, duck, porcine meat, beef and mutton), honey, milk, intestine.
Sensitivity
SampleAmount
Tissue0.1 ppb
Liver0.1 ppb
Honey, milk0.1 ppb
Intestine0.1 ppb
Specificity
SubstanceCross Reactivity Rate
AMOZ100%
AHD<0.1%
AOZ<0.1%
SEM<0.1%
Assay InfoAssay Methodology: Competitive Inhibition ELISA
Alias SymbolsAMOZ, SEMICARBAZIDE HYDROCHLORIDE, 563-41-7, Semicarbazide HCl, Aminourea hydrochloride, Hydrazinecarboxamide hydrochloride, Semicarbazide chloride
Protein NameSemicarbazide hydrochloride
Description of TargetSnow white crystals. Used as a reagent for ketones and aldehydes with which it affords crystalline compounds having characteristic melting points.
Molecular Weight111.53084 g/mol