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> Prostaglandin E2 ELISA Kit

Prostaglandin E2 ELISA Kit

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Katalog-Nummer OKEH02544

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02544
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Prostaglandin E2 ELISA Kit (OKEH02544) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Prostaglandin E2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Prostaglandin E2 and incubated. The Prostaglandin E2 found in the sample or standards competes with the biotinylated Prostaglandin E2 for limited binding sites on the immobilized anti-Prostaglandin E2 antibody. Excess unbound biotinylated Prostaglandin E2 and sample or standard Prostaglandin E2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Prostaglandin E2 captured in the well and inversely proportional to the amount of Prostaglandin E2 which was contained in the sample or standard.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 87%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 6.2 (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Prostaglandin E2 Microplate96 Wells (12 x 8 Well strips)
Prostaglandin E2 Lyophilized Standard2 x 1,000 pg
Sample Diluent1 x 20 mL
100X Prostaglandin E2-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: PGE2
Target Name: Prostaglandin E2
::Pubchem: 5280360
::Chemical formula: C20H32O5
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant General Prostaglandin E2
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsPGE2, Prostaglandin E2, PGE2, dinoprostone
Protein NameProstaglandin E2
Description of TargetThe naturally occurring prostaglandin E2 (PGE2) is known in medicine as dinoprostone. It has important effects in labour (softens cervix and causes uterine contraction) and also stimulates osteoblasts to release factors that stimulate bone resorption by osteoclasts. PGE2 is also the prostaglandin that ultimately induces fever. Like other prostaglandins, dinoprostone can be used as an abortifacient. It is a direct vasodilator, relaxing smooth muscles, and it inhibits the release of noradrenaline from sympathetic nerve terminals. It does not inhibit platelet aggregation, where PGI2 does. It works by binding and activating the prostaglandin E2 receptor.