Platelet Activating Factor ELISA Kit
Katalog-Nummer OKCD02282
Size : 96Wells
Marke : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for Platelet Activating Factor ELISA Kit (OKCD02282) |
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Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 2h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Platelet Activating Factor (PAF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Platelet Activating Factor (PAF) and unlabeled Platelet Activating Factor (PAF) (Standards or samples) with the pre-coated antibody specific to Platelet Activating Factor (PAF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Platelet Activating Factor (PAF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Platelet Activating Factor (PAF) in the sample. | ||||||||||||||||||||||
ELISA Kit Range | 37.0-3,000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Activating Factor (PAF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Activating Factor (PAF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | CAS: 74389-68-7 PubChem: 108156 | ||||||||||||||||||||||
Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
Sensitivity | 14.7 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Competitive Inhibition Immunoassay |
Gene Symbol | PAF |
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Alias Symbols | Platelet activating factor; C16-PAF; 1-O-Hexadecyl-2-acetyl-sn-glycero-3-phosphocholine; Hag-PC; 1-O-Hexadecyl-platelet-activating factor |
Protein Name | Platelet Activating Factor |