PAFA ELISA Kit

Katalog-Nummer OKEH02543

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PAFA ELISA Kit (OKEH02543) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-PAFA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PAFA and incubated. The PAFA found in the sample or standards competes with the biotinylated PAFA for limited binding sites on the immobilized anti-PAFA antibody. Excess unbound biotinylated PAFA and sample or standard PAFA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PAFA captured in the well and inversely proportional to the amount of PAFA which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 84%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.9% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-PAFA Microplate96 Wells (12 x 8 Well strips)
PAFA Lyophilized Standard2 x 20 ng
Sample Diluent1 x 20 mL
100X PAFA-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: PAFA
Target Name: Platelet-activating factor
::Pubchem: 108156
::Chemical formula: C26H54NO7P
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.09 ng/mL
SpecificityNatural and recombinant General Platelet-activating factor
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsPAFA, Platelet-activating factor, PAFA
Protein NamePAFA
Description of TargetIt is an important mediator of bronchoconstriction. It causes platelets to aggregate and blood vessels to dilate. Thus, it is important to the process of hemostasis. At a concentration of 1E-12 mol/L, PAF causes life threatening inflammation of the airways to induce asthma like symptoms. Toxins such as fragments of destroyed bacteria induce the synthesis of PAF, which causes a drop in blood pressure and reduced volume of blood pumped by the heart, which leads to shock and possibly death. Platelet-activating factor, also known as a PAF, PAF-acether or AGEPC (acetyl-glyceryl-ether-phosphorylcholine) is a potent phospholipid activator and mediator of many leukocyte functions, including platelet aggregation and degranulation, inflammation, and anaphylaxis. It is also involved in changes to vascular permeability, the oxidative burst, chemotaxis of leukocytes, as well as augmentation of arachidonic acid metabolism in phagocytes. It is produced in response to specific stimuli by a variety of cell types, including neutrophils, basophils, injured tissue, monocytes/macrophages, platelets, and endothelial cells.